Composite

Part:BBa_K3015007:Design

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-14)


pTheo-RBS-GFP-Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This device was used to test the increase in downstream gene expression after induction with Theophylline.


Source

Synthetic assembled by golden gate cloning

References